This article presents a simple and systematic approach to HPLC method development, beginning with sample preparation and finishing with practical analytical method validation. step 5 - method ...

本研究通过建立11批次粗毛纤孔菌（Inonotus hispidus）的HPLC指纹图谱，结合MTT法评估其对4T1和HeLa细胞的抑制作用，运用灰色关联分析（GRA）和偏最小二乘回归（PLSR）揭示了多组分协同抗肿瘤作用机制，其中峰3、5（hispidin）、6、7为关键活性成分，乙酸乙酯部位富集 ...

Investigation of peak purity failure during HPLC method validation led to discovery of a co-eluting impurity under the main peak. Spectral analysis, including three-dimensional modelling, was used to ...